The TECHSPEC® Fluorescence Filter Set for Cy3 Fluorescence Dye features an excitation filter, an emission filter, and a dichroic filter that complement the Cy3 spectrum. The excitation filter passes wavelengths within the Cy3 excitation/absorption spectrum with a range of 513 - 556nm while the emission filter passes wavelengths within the Cy3 emission spectrum with a range of 570 - 613nm.
我們的 TECHSPEC® 螢光濾光片套件旨在整合到螢光顯微鏡中時提供明亮的影像。帶通濾光片的高穿透率保證了盡可能明亮的影像,而深度阻擋則確保沒有不需要的光到達偵測器。這確保了影像比通常使用的軟塗層蒸鍍濾光片更明亮,並且具有更高的訊號雜訊比。 TECHSPEC® 螢光濾光片組非常適合螢光顯微鏡或螢光成像應用,可用於常見的螢光染料和螢光蛋白。此外,這些過濾器也用於聚合酶鏈反應 (PCR) 診斷儀器。
螢光濾光片組包括來自 TECHSPEC® 螢光帶通濾光片的激發和發射濾光片以及來自 TECHSPEC® 二向色濾光片的二向色濾光片。激發濾光片放置在螢光顯微鏡的照明路徑內,以僅透過所檢查螢光團激發範圍內的波長。發射濾光片放置在螢光顯微鏡的成像路徑內,僅通過螢光團發射範圍內的波長。二向色濾光片放置在激發濾光片和發射濾光片之間,以將激發訊號反射向螢光團,同時將發射訊號傳送到偵測器。
濾光片組列出了最常見的螢光團。有關每組相容螢光團的完整列表,請查看螢光顯微鏡的螢光團和濾光片。 TECHSPEC® 螢光濾光片組可預先安裝在Olympus或Nikon濾光片立方體中。
Excitation and Emission Filters | Dichroic Filter | |
Size | 25.0mm | 25.2 x 35.6mm |
Thickness | 3.5mm | 1.05mm |
Tolerances | ||
Size | +0.0/-0.1 | |
Thickness | ±0.1mm | |
Clear Aperture | 22.0mm | 22.0 x 30.0mm |
Transmitted Wavefront (RMS) | <λ/4 | |
Angle of Incidence | 0.0° ±5.0° | 45.0° ±5.0° |
Compatible Microscopes | Most common Nikon, Olympus, and Zeiss Microscopes |
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