535nm CWL, 12.5mm Dia, 43nm Bandwidth, OD 6 Fluorescence Filter

Product Description

The TECHSPEC® 535nm CWL, 12.5mm Dia, 43nm Bandwidth, OD 6 Fluorescence Filter can be used as a Cy3 FITC filter to pass the excitation spectrum of Cy3 or the emission spectrum of FITC.

產品系列說明

• 適用於常見螢光團的常見波長
• 可選擇激發和發射濾光片
• 穿透率 >93%
• 截止 >OD 6，距離頻寬邊緣<3%。
• 另有螢光濾光片套件 和 預安裝的螢光濾光片立方體組件 可供選擇

TECHSPEC® 螢光帶通濾光片與螢光成像應用中的常見螢光應用完美匹配。激發濾光片和發射濾光片具有>93%的穿透率和>OD 6的波段外攔截。這些濾光片也是光譜學和臨床化學應用以及生物技術儀器（如：DNA定序儀和聚合酶連鎖反應（PCR）測試平臺）的理想組件。每個濾光片使用加硬濺鍍技術製成，安裝在一個黑色的陽極處理環中。

關於每個激發或發射濾光片適用螢光波段應用的完整清單，請查看技術文章《 螢光顯微鏡的螢光和光學濾光片》。

Fluorescence microscopy and spectroscopy applications typically use a combination of three filters for each color channel (detection of individual fluorophores), an excitation and an emission bandpass filter, as well as a dichroic filter (also dichroic beamsplitters, such as our Fluorescence Dichroic Filters and High-Performance Fluorescence Dichroic Filters). More information on fluorescence microscopy and an example beam path can be found in the AppNote Fluorophores and Optical Filters for Fluorescence Microscopy. In short, excitation filters “clean up” the excitation light used to excite the fluorophores in the sample. On the other hand, emission filters allow only the emitted fluorescence to pass through to the detector while effectively blocking the excitation light and different wavelengths, producing high-contrast images and superior image clarity.

Our fluorescence bandpass filters are engineered with cutting-edge technology and are designed to enhance signal-to-noise ratios, improve contrast, and deliver excellent performance in optical imaging applications. These filters are designed to be widely compatible with microscopes from Nikon, Olympus, and Zeiss, making them suitable for various imaging applications, including widefield, confocal, multiphoton, and other fluorescence microscopy modalities. Whether you are imaging fixed cells, live cells, or tissues, our filters are engineered to deliver consistent and reliable image quality. Additionally, these filters can be used for fluorescence detection in qPCR, flow cytometry, or multi-well plate readers in applications such as immunofluorescence or high-throughput screening in which fluorescent molecules or dyes detect specific molecular structures or DNA sequences.

TECHSPEC® Fluorescence Bandpass Filters are optimized for maximum transmission within a specific wavelength range. They effectively isolate fluorescence signals from background noise, enhancing sensitivity and enabling more accurate detection of fluorophores. Explore our range of filters today and unleash the full potential of your fluorescence microscopy setup.