Name: 500nm CWL, 25mm Dia, 24nm Bandwidth, OD 6 Fluorescence Filter
Brand: TECHSPEC
SKU: 67-029
Price: NT$11,445

500nm CWL, 25mm Dia, 24nm Bandwidth, OD 6 Fluorescence Filter

Specifications

概況

Type:

Bandpass Filter

Compatible Fluorophore:

YFP Excitation

物理和機械特性

Diameter (mm):

25.00 +0.00/-0.10

Clear Aperture CA (mm):

21.21

Thickness Tolerance (mm):

±0.1

Construction:

Mounted in Black Anodized Ring

Physical Durability:

Adhesion per MIL-PRF-13830B, Section C.4.5.12
Moderate abrasion per MIL-PRF-13830B, Section C.4.5.11
Cleaning per MIL-C-48497A Section 4.5.4.2

Substrate Thickness (mm):

2.00 ±0.25

光學特性

Angle of Incidence (°):

0 ±5

Bandwidth (nm):

24.00

OD 6 Blocking Wavelength Range (nm):

250 - 473 & 525 - 670

Optical Density OD (Average):

≥6.0

Average Transmission (%):

>93 over Bandwidth

Center Wavelength CWL (nm):

500.00

Full Width-Half Max FWHM (nm):

29.00

Fused Silica (Corning 7980)

Coating:

Hard Coated

Surface Quality:

60-40

Transmission (%):

93.00

Blocking Wavelength Range (nm):

250 - 1100

Transmitted Wavefront, RMS:

λ/4 (prior to coating)

螺紋和安裝

Mount Thickness (mm):

3.5 ±0.1

環境和耐用性因素

Environmental Durability:

Humidity per MIL-STD-810H, Section 507.6
Temperature per MIL-STD-810H, Section 501.7 and 502.7

Regulatory Compliance

RoHS 2015:

Compliant

Certificate of Conformance:

View

REACH 241:

Compliant

產品概覽

適用於常見螢光團的常見波長
可選擇激發和發射濾光片
穿透率 >93%
截止 >OD 6，距離頻寬邊緣<3%。
另有螢光濾光片套件和預安裝的螢光濾光片立方體組件可供選擇

TECHSPEC® 螢光帶通濾光片與螢光成像應用中的常見螢光應用完美匹配。激發濾光片和發射濾光片具有>93%的穿透率和>OD 6的波段外攔截。這些濾光片也是光譜學和臨床化學應用以及生物技術儀器（如：DNA定序儀和聚合酶連鎖反應（PCR）測試平臺）的理想組件。每個濾光片使用加硬濺鍍技術製成，安裝在一個黑色的陽極處理環中。

關於每個激發或發射濾光片適用螢光波段應用的完整清單，請查看技術文章《螢光顯微鏡的螢光和光學濾光片》。

Fluorescence microscopy and spectroscopy applications typically use a combination of three filters for each color channel (detection of individual fluorophores), an excitation and an emission bandpass filter, as well as a dichroic filter (also dichroic beamsplitters, such as our Fluorescence Dichroic Filters and High-Performance Fluorescence Dichroic Filters). More information on fluorescence microscopy and an example beam path can be found in the AppNote Fluorophores and Optical Filters for Fluorescence Microscopy. In short, excitation filters “clean up” the excitation light used to excite the fluorophores in the sample. On the other hand, emission filters allow only the emitted fluorescence to pass through to the detector while effectively blocking the excitation light and different wavelengths, producing high-contrast images and superior image clarity.

Our fluorescence bandpass filters are engineered with cutting-edge technology and are designed to enhance signal-to-noise ratios, improve contrast, and deliver excellent performance in optical imaging applications. These filters are designed to be widely compatible with microscopes from Nikon, Olympus, and Zeiss, making them suitable for various imaging applications, including widefield, confocal, multiphoton, and other fluorescence microscopy modalities. Whether you are imaging fixed cells, live cells, or tissues, our filters are engineered to deliver consistent and reliable image quality. Additionally, these filters can be used for fluorescence detection in qPCR, flow cytometry, or multi-well plate readers in applications such as immunofluorescence or high-throughput screening in which fluorescent molecules or dyes detect specific molecular structures or DNA sequences.

TECHSPEC® Fluorescence Bandpass Filters are optimized for maximum transmission within a specific wavelength range. They effectively isolate fluorescence signals from background noise, enhancing sensitivity and enabling more accurate detection of fluorophores. Explore our range of filters today and unleash the full potential of your fluorescence microscopy setup.

Technical Information

All mounted TECHSPEC® Optical Filters have an arrow on the side of the mount that points to the filter-coated surface for quick reference. Filter oriented such that arrow points to filter coated surface S1. Anti-reflective (AR) coating is applied to S2.

Accessories

成像鏡頭選擇器

Frequently Purchased Together

#02-105 - 12.5mm Diameter Float Glass Window

NT$473

#33-324 - 512nm CWL, 25mm Dia, 25nm Bandwidth, OD 6 Fluorescence Filter

NT$11,445

$¼-20 Mounting Adapter for IDS Imaging USB 3/GigE/PoE Camera - Rev 2$

#35-088 - ¼-20 Mounting Adapter for IDS Imaging USB 3/GigE/PoE Camera - Rev 2

NT$1,400

#47-207 - 1.0 OD 25mm Diameter UV-VIS ND Filter

NT$4,200

Compatible Mounts

Title	Type	庫存號碼	價格	Buy
25.0/25.4mm Optic Dia., SM1 Thin Mount, M4	Fixed	#13-787	NT$683 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., SM1 Thin Mount, 8-32	Fixed	#13-788	NT$683 Request Quote	5-7 Days ×
25.0mm Optic Dia., Optic Mount	Fixed	#64-560	NT$1,068 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., E-Series Kinematic Mount	Adjustable - Tip-Tilt	#15-866	NT$1,383 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., SM1 Thin Mount, M4, Quick Start Optical Mounts	Fixed	#17-536	NT$2,083 Request Quote	聯絡我們 ×
25.0/25.4mm Optic Dia., SM1 Thin Mount, 8-32, Quick Start Optical Mounts	Fixed	#17-537	NT$2,083 Request Quote	聯絡我們 ×
2.0 - 35.0mm Optic Dia., Three-Screw Adjustable Ring Mount	Fixed	#03-668	NT$2,170 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., L-Slot Direct Mount	Fixed	#36-410	NT$2,328 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., Side Flange Direct Mount	Fixed	#36-414	NT$2,328 Request Quote	5-7 Days ×
9.5 - 73.0mm Optic Dia., Three-Screw Adjustable Ring Mount	Fixed	#36-605	NT$2,713 Request Quote	聯絡我們 ×
25/25.4mm Diameter, E-Series Kinematic Mount, Quick Start Optical Mounts	Adjustable - Tip-Tilt	#17-521	NT$2,783 Request Quote	聯絡我們 ×
25.0/25.4mm Optic Dia., Kinematic Mount, 2-Screws	Adjustable - Tip-Tilt	#58-851	NT$3,185 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., L-Slot and Rotation Direct Mount	Adjustable - Rotary	#36-411	NT$3,325 Request Quote	5-7 Days ×
5.0 - 25.0mm Optic Height, Metric Bar-Type Optic Holder	Fixed	#55-529	NT$3,325 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., Kinematic Mount, 3-Screws	Adjustable - Tip-Tilt	#58-854	NT$3,465 Request Quote	5-7 Days ×
7.0 - 40.0 Optic Height, English Bar-Type Optic Holder	Fixed	#03-676	NT$3,465 Request Quote	5-7 Days ×
10.0 - 60.0mm Optic Height, Metric Bar-Type Optic Holder	Fixed	#55-530	NT$3,535 Request Quote	5-7 Days ×
19.5 - 108.0mm Optic Dia., Three-Screw Adjustable Ring Mount	Fixed	#03-670	NT$3,710 Request Quote	5-7 Days ×
7.0 - 67.0 Optic Height, English Bar-Type Optic Holder	Fixed	#03-669	NT$3,780 Request Quote	5-7 Days ×
8.0 - 118.0 Optic Height, English Bar-Type Optic Holder	Fixed	#03-666	NT$3,920 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., Optical Cell Assembly	Fixed	#36-461 Clearance Clearance Availability is limited, so buy before these products disappear. Sorry, we cannot accept returns. Receive up to 80% off select products.	NT$4,830 Request Quote	5-7 Days ×
25/25.4mm Dia., Kinematic Mount, 3-Screws, Quick Start Optical Mounts	Adjustable - Tip-Tilt	#17-523	NT$4,865 Request Quote	聯絡我們 ×
4.0 - 36.0mm Optic Dia., Self-Centering Jaw Clamp	Fixed	#16-077	NT$5,145 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., Stainless Steel Kinematic Mount, 2-Screws	Adjustable - Tip-Tilt	#26-815 New New Launched on our website before appearing in our latest print catalogs. Be the first to buy before anyone else!	NT$5,250 Request Quote	聯絡我們 ×
2"/50.8mm Diameter, Bar Type Lens / Filter Holder, Quick Start Optical Mounts	Fixed	#17-530	NT$5,259 Request Quote	聯絡我們 ×
25.0/25.4mm Optic Dia., Stainless Steel Kinematic Mount, 3-Screws	Adjustable - Tip-Tilt	#26-818 New New Launched on our website before appearing in our latest print catalogs. Be the first to buy before anyone else!	NT$5,950 Request Quote	聯絡我們 ×
25.0/25.4mm Optic Dia., X-Y Translating Optic Mount	Adjustable - Linear (XY)	#62-956	NT$9,030 Request Quote	5-7 Days ×
25.0/25.4mm Optic Diameter, X-Y Translating Quick Start Optical Mounts	Adjustable - Linear (XY)	#17-513	NT$10,544 Request Quote	聯絡我們 ×
25.0/25.4mm Optic Dia., Kinematic Mirror Mount	Adjustable - Tip-Tilt	#55-003	NT$10,990 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., Kinematic Mirror Mount with Micrometer	Adjustable - Tip-Tilt	#55-456	NT$11,795 Request Quote	5-7 Days ×
5.0 - 100.0mm Optic Dia., Self-Centering Jaw Clamp	Fixed	#16-078	NT$13,405 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., Kinematic Mirror Mount with Micrometer, Quick Start Optical Mounts	Adjustable - Tip-Tilt	#17-519	NT$13,510 Request Quote	聯絡我們 ×
25.0/25.4mm Optic Dia., Precision Gimbal Mount	Adjustable - Gimbal	#54-999	NT$15,050 Request Quote	5-7 Days ×
25/25.4mm ID, Precision Gimbal Mount, Quick Start Optical Mounts	Adjustable - Gimbal	#17-517	NT$16,914 Request Quote	聯絡我們 ×
25.0/25.4mm Optic Dia., X-Y-Z Translating Optic Mount	Adjustable - Linear (XYZ)	#62-959	NT$17,675 Request Quote	5-7 Days ×
25.0/25.4mm Optic Dia., 5 Axes Optical Mount	Adjustable - Linear (XYZ) & Tip-Tilt	#13-776	NT$24,675 Request Quote	聯絡我們 ×

Check out our full selection of mounts here.

資源

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500nm CWL, 25mm Dia, 24nm Bandwidth, OD 6 Fluorescence Filter

Specifications

概況

物理和機械特性

光學特性

螺紋和安裝

環境和耐用性因素

Regulatory Compliance

產品概覽

Technical Information

All mounted TECHSPEC® Optical Filters have an arrow on the side of the mount that points to the filter-coated surface for quick reference. Filter oriented such that arrow points to filter coated surface S1. Anti-reflective (AR) coating is applied to S2.

Accessories

相關產品

成像鏡頭選擇器

Frequently Purchased Together

Compatible Mounts

資源

Confocal Microscopy

使用短波通與長波通濾光片的製作自訂帶通濾光片

The Importance of Optical Filter Orientation, Angle of Incidence, and Cone Half Angle

Optical Filters Review

濾光片鍍膜-傳統鍍膜和硬質濺鍍膜的比較

Selecting Color Filter Glass for Life Science Applications

What type of material should I look for in a filter?

How are notch filters designed and manufactured?

Bandpass Filter

Bandwidth

Cavity

Dichroic Coating

Dichroic Filter

Filter, Colored Glass

Ripple

光學濾光片的未來

顯微鏡用螢光濾光片

光學濾光片

機器視覺濾光的技術

Polarizers Review

Manipulating Wavebands: Color & Filters

What happens when a filter is tilted?

How do I determine which side of my filter is coated?

Blocking

Blocking Range

Central Wavelength (CWL)

Cold Mirror

Full Width at Half Maximum (FWHM)

Interference Filter

Notch Filter

Peak Transmittance

Rugate Notch Filter

Shortpass Filter

螢光成像搭配雷射照明

How do I clean my filters?

What is the largest diameter filter available from Edmund Optics®?

What are some of the most common filter substrates?

How do I select the right filter for my application?

What types of filters can Edmund Optics® manufacture?

Do you have mounts for square or rectangular optics?

Cut-Off Wavelength

Cut-On Wavelength

Filter

Fluorescent Filter

Optical Density (OD)

Stopband

Optical Microscopy Application: Fluorescence

Basic Principles of Raman Scattering and Spectroscopy

Fluorescence Microscopy: In-Line Illumination with Imaging Filters

Removing Protective Plastic Coating

Parallelism

Surface Flatness

How do I clean my optics?

Clear Aperture (CA)

愛特蒙特光學遍佈全球的製造工廠

愛特蒙特光學的度量技術：將測量作為製造的關鍵要素